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NC-19 induces HT-29 G0/G1 phase cell cycle arrest and ROS generation. (A and B) Cell cycle analysis of HT-29 cell cycle distribution after treatment with NC-19 (0, 2, 4, 8 µM) for 24 hours. (C and D) Western blot and bar graph analysis of <t>CDK2</t> and Cyclin E protein expression in HT-29 cells after treatment with NC-19 (0, 5, 10, 20 µM) for 24 hours. (E) Cell viability of HT-29 cells pretreated with NAC (2 mM) for 8 hours, followed by incubation with or without 2 µM NC-19 for 72 hours. (F) Detection of ROS generation by DCFH-DA in HT-29 cells after treatment with NC-19 (0, 1, 2, 5, 10 µM) for 24 hours.
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NC-19 induces HT-29 G0/G1 phase cell cycle arrest and ROS generation. (A and B) Cell cycle analysis of HT-29 cell cycle distribution after treatment with NC-19 (0, 2, 4, 8 µM) for 24 hours. (C and D) Western blot and bar graph analysis of <t>CDK2</t> and Cyclin E protein expression in HT-29 cells after treatment with NC-19 (0, 5, 10, 20 µM) for 24 hours. (E) Cell viability of HT-29 cells pretreated with NAC (2 mM) for 8 hours, followed by incubation with or without 2 µM NC-19 for 72 hours. (F) Detection of ROS generation by DCFH-DA in HT-29 cells after treatment with NC-19 (0, 1, 2, 5, 10 µM) for 24 hours.
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NC-19 induces HT-29 G0/G1 phase cell cycle arrest and ROS generation. (A and B) Cell cycle analysis of HT-29 cell cycle distribution after treatment with NC-19 (0, 2, 4, 8 µM) for 24 hours. (C and D) Western blot and bar graph analysis of CDK2 and Cyclin E protein expression in HT-29 cells after treatment with NC-19 (0, 5, 10, 20 µM) for 24 hours. (E) Cell viability of HT-29 cells pretreated with NAC (2 mM) for 8 hours, followed by incubation with or without 2 µM NC-19 for 72 hours. (F) Detection of ROS generation by DCFH-DA in HT-29 cells after treatment with NC-19 (0, 1, 2, 5, 10 µM) for 24 hours.

Journal: RSC Advances

Article Title: β-caryolane derivatives as novel anti-colorectal cancer agents: synthesis and in vitro biological evaluation

doi: 10.1039/d5ra07312j

Figure Lengend Snippet: NC-19 induces HT-29 G0/G1 phase cell cycle arrest and ROS generation. (A and B) Cell cycle analysis of HT-29 cell cycle distribution after treatment with NC-19 (0, 2, 4, 8 µM) for 24 hours. (C and D) Western blot and bar graph analysis of CDK2 and Cyclin E protein expression in HT-29 cells after treatment with NC-19 (0, 5, 10, 20 µM) for 24 hours. (E) Cell viability of HT-29 cells pretreated with NAC (2 mM) for 8 hours, followed by incubation with or without 2 µM NC-19 for 72 hours. (F) Detection of ROS generation by DCFH-DA in HT-29 cells after treatment with NC-19 (0, 1, 2, 5, 10 µM) for 24 hours.

Article Snippet: The primary antibodies were as follows: caspase-3 (CST, 14220T), cleaved caspase-3 (CST, 9664T), PARP1 (CST, 9532T), cleaved PARP1 (CST, 5625T), GAPDH (CST, 2118T), CDK2 (Proteintech, 10122-1-Ap), cyclin E (Proteintech, 11554-1-Ap), MMP9 (Proteintech, 10375-2-Ap), calnexin (Proteintech, 10427-1-Ap), tubulin (Proteintech, 10094-1-Ap).

Techniques: Cell Cycle Assay, Western Blot, Expressing, Incubation